Aras Part Medical International Press International Journal of Medical Parasitology and Epidemiology Sciences 2766-6492 7 3 2026 09 18 In Silico Analysis and Immunogenicity Assessment of the Toxoplasma gondii Dense Granule Antigen 7 (GRA7) 171 182 10.34172/ijmpes.6282 EN Siti Fadilla https://orcid.org/0009-0002-9370-1027 Muhammad Luqman Habibi https://orcid.org/0009-0001-9510-7788 Nuning Winaris https://orcid.org/0000-0001-5941-7095 Loeki Enggar Fitri https://orcid.org/0000-0002-4880-1048 Journal Article 10.34172/ijmpes.6282 2026 06 17 2026 07 30 Introduction: Toxoplasma gondii is a parasite of the protozoan type and is intracellular. These parasites can cause the occurrence of toxoplasmosis in infected individuals. These protozoa are zoonotic and have a global prevalence. The method of enforcing diagnosis in this parasite serologically, molecularly, and microscopically has limitations, namely in the values of sensitivity, specificity, and feasibility. Dense Granule Antigen 7 (GRA 7), which is a protein secreted by T. gondii during the infection stage in the acute and chronic phases, can elicit a strong immune response, so GRA 7 can be used as a biomarker and is very promising. Methods: This study used a method in the form of in silico. This method was performed to characterize the physiochemical properties, secondary and tertiary structures, post-translational modification, and immunogenic epitopes of GRA7. Results: In silico analysis found that GRA7 is hydrophilic. In terms of structural stability, GRA 7 is dominated by alpha-helix. Furthermore, GRA7 contains a transmembrane domain and several predicted epitope regions that may be recognized by B cells and T cells. All of these findings support that GRA7 has the potential to be a potent antigen. In addition, the recombinant productivity of GRA7 increases its application as a diagnostic and on a wide scale. Conclusions: Overall, GRA7 shows strong potential as a reliable antigen for serological diagnosis of toxoplasmosis due to its Received: xx xx, 2026, Revised: xx xx, 2026, Accepted: x 13, 2026, ePublished: xx xx, 2026 high sensitivity and specificity, especially among vulnerable groups such as pregnant women and individuals with weakened immune systems.